Google is appealing the European Commission’s decision to fine it a record £2bn for breaching antitrust rules.
The search giant was accused earlier this year of prioritising its own shopping service in search results, prompting regulators to issue the largest antitrust fine in EU history.
Google outlined its plans to comply with the order last month in a move that signalled the beginning of a more conciliatory relationship between the two organisations.
But the appeal is now set to lead to a drawn out court battle that is likely to reignite tensions.
The Californian firm will still have to pay the fine, but it can deposit it in a blocked account until the end of the case.
Alphabet, Google’s parent company, faces further fines of up to 5 per cent of daily global turnover if it fails to comply with the ruling by 28 September.
Margrethe Vestager, the European antitrust commissioner, told French news agency AFP last week that Google’s plans to meet EU rules “pointed in the very right direction”.